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细胞学制备技术对RNA质量的影响:一项关于涂片样本的比较研究
细胞学制备技术对RNA质量的影响研究。通过比较MGG、PAP、Diff-Quik和空气干燥4种技术对182例手术标本RNA质量的影响,发现MGG染色(ΔCt最高且最稳定)最佳,PAP最差(ΔCt最低),空气干燥结果最不稳定。RNA浓度差异不显著(p=0.07),但RNA质量直接影响qPCR和NGS等分子 ...
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