Normalization of RNA-sequencing (RNA-seq) data has proven essential to ensure accurate inference of expression levels. Here, we show that usual normalization approaches mostly account for sequencing ...

The normalization of RNA-seq data is essential for accurate downstream inference, but the assumptions upon which most normalization methods are based are not applicable in the single-cell setting.

This is the second in a series on what’s still broken in the analytics space. Part one dealt with data ownership, part two will address technology and part three will focus on people and processes.