VEN123L Video 9.1 - Limits of Detection and Quantification
ucdavis.edu
VEN123L Video 9.1 - Limits of Detection and Quantification
What's the lowest concentration that a method or instrument can detect, and what's the lowest it can measure? What's the difference? How do we determine the limits of detection and quantification?Copyright Thi NguyenDo not distribute without permissionthinguy@ucdavis.edu
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SOLVED: Detection limit. In spectrophotometry, we measure the concentration of analyte by its absorbance of light. A low concentration sample was prepared, and nine replicate measurements gave absorbances of 0.0047, 0.0054, 0.0062, 0.0060, 0.0046, 0.0056, 0.0052, 0.0044, and 0.0058 in a 1.000-cm cell. Nine reagent blanks gave values of 0.0006, 0.0012, 0.0022, 0.0005, 0.0016, 0.0008, 0.0017, 0.0010, and 0.0011.(a) Find the absorbance detection limit with Equation 5-2.(b) The calibration curve is
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