Draw a restriction map for a recombinant plasmid that includes ... | Filo

SOLVED: The figure below shows a restriction map of a piece of DNA containing your favorite gene. The arrow indicates the position and orientation of the gene in the DNA. In part (B) of the figure are enlargements showing the portions of the DNA whose sequences have been used to make oligonucleotide probes A, B, C, and D. Which of the oligonucleotides can be used to detect the gene in each of the following?(A)(B)A. A Southern blot of genomic DNA cut with HindIII.B. A Northern blot.

a. Given the following restriction map of a cloned 10 kb piece of DNA, what size fragments would you see after digesting this linear DNA fragment with each of the enzymes or combinations of enzymes listed? (1) EcoRI, (2) BamHI, (3) EcoRI + HindIII, (4) BamHI + Pstl, and (5) EcoRI + BamHI. b. What fragments in the last three double digests would hybridize on a Southern blot with a probe made from the 4 kb Bam HI fragment? | Numerade